Piscine nodaviruses are members of genus Betanodavirus, which infects a large number of species of fish and causes massive mortality in larvae and juveniles. The disease causes great economic losses in aquaculture and sea-ranching. Virus-like particles (VLPs) of dragon grouper nervous necrosis virus (DGNNV) were employed to screen the consensus sequences of paratopes by the phage display peptide
technology. Using two biopanning methods to screen phages, their binding efficiency was examined by ELISA. The effects of treatment by different blocking buffers on the ELISA assays and the effects of the ELISA plates exposed to UV light were tested. The display phages with
high binding efficiency were sequenced after the inserted fragment by PCR amplification. With NCBI BLAST, they were closely related to the claudin family and the consensus sequences were on the region of EL1 or
EL2. Few non-claudin sequences demand further comparison with other kinds of receptors to ascertain the characters of these sequences.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0908110-155907 |
| Date | 08 September 2010 |
| Creators | Lin, Cheng-Long |
| Contributors | Chi-Hsin Hsu, Chan-Shing Lin, Zhi-Hong Wen |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908110-155907 |
| Rights | not_available, Copyright information available at source archive |
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