Population genetic variation of cultured oyster Crassostrea spp. in Taiwan and neighborhood
Cheng-Pin Yu
¡]Advisor¡GDr. Jin-Hua Cheng¡^
Institute of Marine Biology, National Sun Yat-sen University, Kaohsuang 804 Taiwan R.O.C.
Thesis Abstract
The genetic variations among oyster populations of Taiwan, Ginmen and Fu-kein coast are analyzed using molecular method. Firstly, DNA sequences of mitochondrial 16S rRNA and COI genes are analyzed according to nucleotide differences and restriction enzyme recognition site. Then, population variations were analyzed by the lane pattern of restriction enzyme fragment¡]RFLP¡^.
The sequences of 530 bp DNA fragment of 16S rRNA gene showed little difference¡]0-1 bp¡^between Portuguese oyster and oyster cultured in Taiwan, both have larger differences¡]3-4 bp¡^compared with C. gigas. The sequences of 700 bp DNA fragment of COI gene showed similar results. C. angulata is found in Ginmen and Fu-kein coast for the first time which is identified by the PCR-RFLP of COI gene fragment. PCR-RFLP analysis of COI gene fragment showed C. angulata from Portugal are genetically closest to the oysters collected from Ginmen and Fu-kein.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0910102-163146 |
| Date | 10 September 2002 |
| Creators | Yu, Cheng-Pin |
| Contributors | Tzyy-Ing Chen, Jin-Hua Cheng, Li-Lian Liou |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0910102-163146 |
| Rights | unrestricted, Copyright information available at source archive |
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