According to GenBank, ROGDI is located on chromosome
16p13.3 and the size of its coding region is 864 bp which encodes
287 amino acids. It is a novel gene which has unknown function. By
bioinformatic analysis, the product of this gene was predicted as a hydrophilic protein containing leucine zipper domain. Some transcription factors utilize their leucine zipper structures as function domains. A full-length coding region cDNA of this gene was cloned in our laboratory. After the ROGDI gene was transfected into HEK293T cells, cell clones with different ROGDI protein expression levels were selected and classified into groups of high, middle and low. According to the results of foci formation assay, growth curve, anchorage indepentdent growth assay and MTS assay, it is found that clones with higher ROGDI protein level could enhance faster proliferation rate of HEK293T cells. Thus ROGDI may be a positive regulator of cell cycle and may play a role in cell proliferation.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0913107-140635 |
| Date | 13 September 2007 |
| Creators | Tseng, Chia-yin |
| Contributors | Pei-Jung Lu, Chung-Lung Cho, HONG-YO KANG, YI-REN HONG |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0913107-140635 |
| Rights | not_available, Copyright information available at source archive |
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