The abundance and low fat content of Pacific whiting
support Its use for the production of surimi. The
degradation of muscle proteins by myxosporidian secreted
proteinase(s) has been associated with its soft texture.
High residual activity is retained through the washing
process used in the production of surimi and precludes the
formation of a strong heat-set gel by surimi sols.
Physical, chemical and SDS-PAGE analysis defined the
reinforced oxidation of free sulfhydryl groups on
myofibrillar proteins to disulfide bonds by potassium
bromate. SDS-PAGE demonstrated myosin degradation during
heat-setting and the protection of myosin from protelnase
attack by bromate. A level of 0.075% bromate Inactivated
89.87% of the total proteinase activity in sols. It was
assumed that cysteine proteinases were Inactivated and residual activity was associated with proteinases with a
serine active site.
Major iraprovement in gel coheslveness and elasticity
was observed at bromate levels [less than or equal to] 0.075% with only a slight
improvement at higher levels. Maximum hardness was observed
at 0.150% with no (P>0.050) increase at higher levels.
Brittleness was improved (P>0.050) by bromate levels
[greater than or equal to] 0.100%; no maximum degree of brittleness was observed
within the range ([less than or equal to] 0.250%) of concentrations investigated.
An optimum folding test grade of AA was achieved by a
minimum of 0.150%
Potassium bromate improved gelling characteristics of
sols of Pacific whiting surimi through proteinase
inactivation and reinforced disulfide formation during
heat-setting. Improvement in cohesiveness and elasticity
was primarily a function of proteinase inactivation.
Maximum hardness and brittleness required additional
oxidative capacity which was not fully required for an
optimum folding test grade. / Graduation date: 1989
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/26197 |
| Date | 03 March 1989 |
| Creators | Aguilar, Ramon Pacheco |
| Contributors | Crawford, David L. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
Page generated in 0.0015 seconds