Achromobacter A43 produced a compound inhibitory to the outgrowth
of Clostridium botulinum type E spores. The inhibitor could
be produced in various laboratory media and the outgrowth of germinated
spores was completely inhibited by 1/10th dilution of the A43
spent medium. Germination was not affected. The spores lost
refractility in the presence of the inhibitor and were darkly stained by
crystal violet. The germinated spores showed little outgrowth, no
elongation, and no lysis.
The A43 inhibitor was dialysable and could be concentrated by
lyophilizing the dialysate. The inhibitor was stable at 37°C, 25°C,
and 5°C, but was partly inactivated when heated at 65°C for 10 min.
The inhibitor was not volatile and could not be vacuum distilled at
40°C. Solutions of acids of pH below 2.0 destroyed the activity.
Molecular weight of the inhibitor was estimated at 800 ~ 1,000 Daltons by PSAC Millipore Pellican ultrafiltration and by elution time
on a column of Bio-Gel P-2. The inhibitor could be separated into
fractions containing peptides and lipids on a Bio-Rex 70 X ion exchange
column. The presence of phosphatidic amino acids was also
suggested by Rhodamine 6G reaction.
The A43 inhibitor was similar, in molecular weight and inhibition
characteristics, to tylosin, but appeared to be more heat labile
than tylosin.
Achromobacter species were shown to be selectively inactivated
by the smoking process. The smoked fish, therefore, may lack the
added safety factor that the inhibitor similar to that of A43 might
provide in other seafoods. / Graduation date: 1974
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/26951 |
| Date | 20 June 1973 |
| Creators | Patrick, Lui-sun Kwan |
| Contributors | Lee, Jong S. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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