Cyclopropenoid fatty acids (CPFA) are natural components of
cottonseed oil, a major food oil in the United States. The ability of
CPFA to cause abnormal biochemical and physiological effects when
fed to laboratory and farm animals has prompted an investigation of
their effects on mice.
Between 0.05 and 0.55% CPFA were fed as glycerines of
Sterculia foetida oil (SFO, containing 55% CPFA) to mice to determine
their effect on certain aspects of growth, lipid metabolism and
mitochondrial function.
One-half percent SFO fed to weanling mice caused a small
temporary decrease in growth rate when compared to controls. Many
mice fed 1% SFO stopped growing and died by the end of a 9-week
feeding trial. Mice fed less than 1% SFO, or 1% SFO for shorter
periods of time, showed increased liver-to-body-weight ratios, accumulation of CPFA in adipose tissue and increased ratios of
16:0/16:1, 18:0/18:1 and total saturated to unsaturated fatty acids in
liver and depot fat. Erythrocytes from CPFA-fed mice hemolyzed
more slowly than erythrocytes from control mice in isotonicnonelectrolytes,
implying an effect of CPFA on membrane lipid composition.
One-half percent SFO fed for 9 to 31 days inhibited almost
completely the incorporation of [¹⁴c] from labeled palmitate or
acetate into liver monounsaturated fatty acids. At the same time,
0.5% SFO retarded the incorporation of label from acetate into
¹⁴C₂ and total liver lipid, but stimulated twofold the incorporation
into liver sterols. The oxidation of labeled palmitate was also
reduced. CPFA caused lipid accumulation in livers.
P:0 ratios of liver mitochondria from mice fed 1% SFO for 6 to
15 days were 1.06- 1.45 while control P:0 ratios were 2.30-2.85.
The decrease was due to decreased phosphorylation, but increased
respiration. The relationship between the observed results and
membrane fatty acid composition was discussed. / Graduation date: 1975
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/26968 |
| Date | 28 June 1974 |
| Creators | Lehman, Michael Wesley |
| Contributors | Nixon, Joseph E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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