A method for separation and characterization of individual anthocyanins was
developed. High Performance Liquid Chromatography (HPLC) with a polymer based
reversed-phase column was used to separate the pigments while on-line Photodiode
Array Detection (PDA) was employed to record the UV and Visible spectrum of the
individual peaks. Spectral information obtained from on-line PDA detection provided
information about: 1) the nature of the aglycone, 2) the sugar substitution pattern
and 3) the presence or absence of hydroxy aromatic organic acids. The nature of the
glycosidation can be determined from the HPLC retention characteristics.
The HPLC/PDA methods were employed to characterize the anthocyanin
profiles of the pigments in cranberry, roselle, cherry, bilberry, grape, red cabbage,
black raspberry, blackberry, elderberry, plum, blackcurrant and strawberry. The
anthocyanidin profiles were also determined for the samples for purposes of
confirmation of the anthocyanin data.
In addition to the anthocyanin and anthocyanidin profiles, the general coloring
properties for most of the samples were also determined. Included were Hunter L, a, b values, total anthocyanin pigment concentration, wavelength maxima, percent
tannin measurements, tinctoral strength, pH measurements and titratable acidity. / Graduation date: 1988
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27165 |
| Date | 17 December 1987 |
| Creators | Hong, Victor |
| Contributors | Wrolstad, Ronald E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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