The bioavailability of vitamin B-6 from four selected
foods was investigated in five men, aged 22 to 25 years,
who were receiving a pyridoxine supplement. The subjects
received a constant diet containing 1.34 mg of vitamin B-6
throughout this five-week study, except on Saturdays and
Sundays when they ate their self-chosen diets. Starting
on day 6 of week 1, following a five-day adjustment period,
the subjects received orally 5-mg crystalline pyridoxine
supplement daily, except on Tuesday and Thursday of each
week. On these two days, the subjects were given orally
0 mg or 2 mg of crystalline pyridoxine, or test doses of
bananas, filberts, soybeans and beef which contained around 2 mg of vitamin B-6. Vitamin B-6 was determined
by microbiological assay with Saccharomyces uvarum.
Vitamin B-6 bioavailability in the test food was determined
by comparing 24-hour urinary total vitamin B-6 in
response to the test food doses to that excreted following
a 2-mg crystalline pyridoxine dose in each subject.
Compared to the 100 percent bioavailability of the 2-mg
crystalline PN dose, the average vitamin B-6 bioavailability
in bananas was 115 + 32% and that in filberts, soybeans
and beef was 93 + 8%, 73 + 20% and 87 + 7%, respectively.
The metabolism of vitamin B-6 in pyridoxine-supplemented
subjects was also investigated by measuring
changes in plasma total vitamin B-6 which increased and
was stablized after three weeks of pyridoxine supplementation.
It was concluded that urinary total vitamin B-6
in pyridoxine-supplemented subjects can be used as a
measure of vitamin B-6 bioavailability from test food doses. / Graduation date: 1983
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27199 |
| Date | 08 December 1982 |
| Creators | Wang, Kuen Wu |
| Contributors | Miller, Lorraine T. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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