This investigation was carried out to characterize
and quantitate the collagen of rockfish intramusclar
connective tissue. Different species ot rocktish were chosen
to represent the range of product quality experienced by the
fish processing industry. Species included widow, a rockfish
noted tor its soft flesh, yellowtail, a rockfish possessing
flesh of intermediatary texture and canary rockfish known
for its firm flesh.
The intramuscular connective tissue collagen was
extracted from white muscle and total, salt, acid and
insoluble collagen content was estimated by hydroxyproline
analysis. No significant difference was observed among species with regard to all collagen fractions. Mean salt,
acid and unsoluble collagen contents were 5.5 %, 8.7 % and
86 %, respectively indicating a relatively highly
crosslinked and stable collagen compared to that reported
for other fish collagens.
The denatured acid soluble collagen traction was
characterized by chromatography on CM-cellulose and the
presence of three different alpha chains was established by
SDS-polyacrylamide gel electrophoresis and amino acid
analysis of the collagen components. The molecular weight
for the alpha chains was estimated to be 96,000 based upon
agarose molecular sieve chromatography. The alpha chains,
designated αl, α2 and α3, had significantly different amino
acid composition with the α3 chain possessing a higher level
of hydroxylated lysine than αl and α2. In general, the amino
acid composition of rockfish muscle collagen was similar to
other reported fish collagens.
Digestion of purified connective tissue and acid
soluble muscle collagen with CNBr and subsequent analysis by
LDS-polyacrylamide slab-gel electrophoresis showed identical
peptide patterns indicating the presence of only one
molecular type of collagen. This evidence along with the
observed ratio of the collagen components resolved by
CM-cellulose chromatography supported a molecular
composition of αl-α2-α3 for rockfish muscle connective
tissue collagen.
Significant difference in skin thickness between species was observed. This correlated with the general
quality ranking of the three species by the fish processing
industry of canary>yellowtail>widow. Skin from canary
rockfish was 38 % thicker than skin from widow rockfish
while yellowtail had a skin of intermediatary thickness.
From this observation, it was concluded that skin thickness
and strength plays a more important role than intramuscular
connective tissue characteristics in determining quality of
product obtainable from the different rockfish species. / Graduation date: 1984
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27427 |
| Date | 09 March 1984 |
| Creators | Bogason, Sigurdur Gretar |
| Contributors | Crawford, David L. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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