The mammalian FMO represents a multigene family which
oxygenates a large number of xenobiotics. No physiological role has
been determined for FMO, although synthesis of disulfide bonds and
detoxification of dietary chemicals have been suggested. Five FMO
gene subfamilies, each containing a single gene, have been identified.
In this study, we determined the patterns of fetal and neonatal
development of FMO1 and FMO2 in rabbit liver and lung. The
expression of two major isoforms, FMO1 and FMO2, in fetal and
neonatal animals were characterized at the steady state levels of
mRNA. Northern and slot blot analyses were performed with cDNA
probes for each isoform to provide a qualitative and quantitative
profile. In order to relate developmental changes in FMO to the metabolism of xenobiotics for which lung is a target organ, the
developmental expression of lung FMO (FMO2) mRNA is compared to
rabbit CYP2B4 and CYP4B1 which are the major constitutive P450s in
lung. The results show that the expression of FMO1 and FMO2 is
tissue-dependent, although the mechanisms controlling the mRNA
expression, such as rate of transcription, processing of primary RNA,
efficiencies of nucleocytoplasmic transport and stability of RNA in
the cytoplasm, are still unknown. The results indicate that the early
development- and tissue-specific expression patterns of mRNA for
FMO1
and FMO2 might play a significant role in the target organ
toxicity of xenobiotics in the rabbit fetus and neonate. / Graduation date: 1995 / Best scan available. Original is a black and white photocopy.
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/28163 |
| Date | 02 December 1994 |
| Creators | Yueh, Mei-Fei |
| Contributors | Williams, David E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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