Tetracycline is a front line antibiotic for the treatment of chlamydial
infections in both humans and animals, and the emergence of tetracycline resistant
(tet[superscript R]) Chlamydia is of significant clinical importance. Recently, several tet[superscript R]
chlamydial strains have been isolated from swine (Sus scrofa) raised in production
facilities in Nebraska. Here, the intracellular development of two C. suis tet[superscript R]
strains, R19 and R27, is characterized through the use of tissue culture and
immunofluorescence. The strains grow in tetracycline up to 4 ��g/ml, while a
tetracycline sensitive (tet[superscript S]) C. suis swine strain (S45) and a C. trachomatis strain of
the human serovar L2 (LGV-434) grow in tetracycline up to 0.1 ��g/ml. Although
inclusions form in the presence of tetracycline, many contain large aberrant RBs
that do not differentiate into infectious EBs. The percentage of inclusions
containing typical developmental forms decreases with increasing tetracycline
concentrations, and at 3 ��g/ml of tetracycline, 100% of inclusions contain aberrant
RBs. However, upon removal of the tetracycline, the aberrant RBs revert to typical
RBs and a productive developmental cycle resumes. In addition, inclusions were
found that contained both C. suis R19 and C. trachomatis L2 after sequential
infection, demonstrating that two biologically distinct chlamydial strains could both
develop within a single inclusion. / Graduation date: 2002
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/32637 |
| Date | 28 June 2001 |
| Creators | Lenart, Jennifer |
| Contributors | Rockey, Daniel D. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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