Recent advances in DNA vaccine technology has brought about a promising
strategy for the control of viruses that contain surface membrane glycoproteins. This type
of vaccine involves the intramuscular injection of a bacterial plasmid containing a gene
encoding a viral protein. The strategy uses eukaryotic processing of the protein as would
naturally occur during a viral infection. In this study, plasmid DNA encoding the
glycoproteins of infectious hematopoietic necrosis virus (pcDNA3-IHNV-g), snakehead
rhabdovirus (pcDNA3-SHRV-g), or spring viremia of carp virus (pcDNA3-SVCV-g) was
injected into the skeletal muscle of rainbow trout fry. At 30 days post-vaccination, fish
were challenged with IHNV. Protection against IHNV was observed among all DNA
vaccinated groups. Fish injected with plasmid pcDNA3-IHNV-g, pcDNA3-SHRV-g, or
pcDNA3-SVCV-g had relative survival rates of 93.2%, 98.3% and 94.9%, respectively.
The mechanisms for the viral mediated resistance induced by these glycoprotein based DNA vaccines is unknown. A parallel study conducted by Dr. Carol Kim on the production of Mx proteins in these fish indicates that the observed protection might be a consequence of the stimulation of interferon. / Graduation date: 1999
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/33623 |
| Date | 08 July 1998 |
| Creators | Drennan, John D. |
| Contributors | Leong, Jo-Ann C. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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