Hop cell wall material (CWM) extracted from hop leaves (hamulus lupulus) was
purified and characterized. The total sugar content, uronic acid content and
monosaccharide composition of the CWM were determined. Galacturonic acid is the
major component in the CWM. A mixture of unsaturated oligogalacturonides were
released from purified hop CWM by autoclaving. The biological activity of these
oligomers was tested for their ability to elicit phytoalexins. The oligomer with
hexagalacturonic acid possessed the greatest biological activity. Column
chromatography and high-pH anion exchange chromatography were used for the sample
separation and purification. Fast-atom-bombardment mass spectrometry (FAB-MS)
was used for the structure elucidation. The FAB-MS spectrum showed that the
unsaturated galacturonosyl residue was located at the nonreducing terminus of the
oligomer. / Graduation date: 1993
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/36614 |
| Date | 03 September 1992 |
| Creators | Su, Hong, 1960 Jan. 1- |
| Contributors | Deinzer, Max L. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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