Three experiments were conducted (1) to develop and characterize
polyclonal antibodies against ovine adipocyte plasma membranes (APM),
(2) to evaluate the effect of administering the developed antibodies to
lambs on lamb growth and carcass characteristics, and (3) to investigate
the possibility of using the developed antibodies to identify antigenic
components on ovine APM that are tissue, species and possibly site
specific. In Experiment 1, the developed antibodies showed a high rate
of reactivity to APM and cross reacted with plasma membranes (PM) from
liver, kidney, heart and erythrocytes. Adsorption of antisera with
either liver PM or erythrocyte PM for 6 h resulted in a significant
reduction in antisera cross-reactivity to liver or erythrocyte PM,
respectively, with little effect on its specific reactivity to APM or
cross-reactivity with PM of the other tissues tested. The antisera also
reacted in different affinities to APM from sheep, pig and rat. The
antisera also showed differential binding to APM isolated from
different anatomical locations. In Experiment 2, five wether lambs were
assigned to either a control or treatment group to study the effect of
passive immunization with the antisera immunoglobulin (ASig) on lamb
growth and carcass characteristics. Treatment with ASig resulted in a
significant (P<.OS) reduction of 45% in wet perirenal fat weight, and of
1.8 and 0.8% in subcutaneous and perirenal lipid content, respectively.
ASig immunization also significantly (P<.O5) reduced blood plasma non-esterified
fatty acid without adversely affecting blood plasma
triglycerides or packed cell volumes (hematocrit). Animals immunized
with ASig also showed a significant (P<.OS) reduction in average weight
daily weight gain, but this effect was not associated with any adverse
effect on efficiency of carcass production. Moreover, passive
immunization tended to improve protein accretion and efficiency of
nitrogen utilization. In Experiment 3, the developed antisera
immunoglobulins recognized three unique ovine APM components, in
comparison with plazma membranes from liver, kidney, heart or RBC, with
molecular weights of 70, 106 and 110 KD, and showed to react more
intensely with APM antigenic components that are in common with PM of
the tissues tested. Antisera immunoglobulins recognized just two (44,
46 KD) antigenic components on porcine APM that are shared with those of
ovine APM but did not react with any component on APM of either rat or
chicken. / Graduation date: 1990
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/38198 |
| Date | 28 September 1989 |
| Creators | Nassar, Abdul-Rahman Hassan |
| Contributors | Hu, Ching Yuan |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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