Aim: Assess the bacterial frencuency of chromogenic pigment, also called “black stain” through the biomolecular technique Polymerase chain reaction (PCR) and the technique automatic sequencing. Methods and materials: In this study was performed the genetical amplification of bacteria strictly isolated after collection of free biofilm dental plaque in 30 deciduous and permanent teeth of 10 patients who presented these pigmentations and were attended at the pediatric dentistry service of the UPC teaching dental clinic. Stata 12.0 statistical software was used for the frequency anda proportion analysis. Results: Bacterial genus Actinomyces was found with higher prevalence 33% (n = 34) Likewise, gender Streptococcus was found in 12.62% (n = 13) in the chromogenic pigmentations. Actinomyces naeslundii was the most frequently isolated bacteria 17.48% (n = 18), within the 103 isolates bacteria identified by the technique of replication and genetic identification. Conclusion: It was possible to identify the bacterial composition of the chromogenic pigmentation by using advanced and accurate biomolecular techniques. However, known the colonizing bacteria, is just the first step in understanding the etiology, because this phenomenom is multifactorial, and more research is required to know the production of these pigments. / Objetivo: Evaluar la frecuencia bacteriana de las pigmentaciones cromógenas, llamadas también “black stain” mediante la técnica de biología molecular Reacción en Cadena de la Polimerasa (PCR) y la técnica secuenciación automática. Materiales y métodos: En este estudio se realizó la amplificación genética de bacterias estrictamente aisladas luego de la recolección del biolfilm libre de placa dental en 30 piezas deciduas y permanentes de 10 pacientes portadores de estas pigmentaciones que acudieron al servicio de odontopediatría de la clínica docente de la UPC. Para el análisis de frecuencia y proporción se usó el software estadístico Stata® 12.0 Resultados: Actinomyces fue el género bacteriano encontrado con mayor prevalencia 33% (n=34) así mismo, fue encontrado el género Streptococcus en un 12.62% (n=13) en las pigmentaciones cromógenas. El Actinomyces Naeslundii fue la bacteria aislada con mayor frecuencia 17.48% (n=18), dentro de las 103 bacterias identificadas por la técnica de replicación e identificación genética. Conclusión: Se logró identificar la composición bacteriana de las pigmentaciones cromógenas mediante el uso de técnicas biomoleculares avanzadas y certeras. No obstante, conocer el tipo bacteria colonizadora, es solo el primer paso para entender la etiología, ya que este fenómeno es multifactorial y se requiere mayor investigación para saber la producción de los pigmentos. / Tesis
| Identifer | oai:union.ndltd.org:PERUUPC/oai:repositorioacademico.upc.edu.pe:10757/337350 |
| Date | 2014 December 1917 |
| Creators | García Ortega, Hanssell Oswaldo |
| Contributors | Mayta Tovalino, Frank Roger, Del Valle Mendoza, Juana Mercedes |
| Publisher | Universidad Peruana de Ciencias Aplicadas (UPC), PE |
| Source Sets | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Language | Spanish |
| Detected Language | Spanish |
| Type | info:eu-repo/semantics/bachelorThesis |
| Format | application/pdf, application/msword |
| Source | Universidad Peruana de Ciencias Aplicadas (UPC), Repositorio Académico UPC |
| Rights | info:eu-repo/semantics/OpenAccess |
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