A panel of metabolic enzyme genetic polymorphisms, which are involved in cigarette carcinogen metabolism, also a DNA repair gene involved in the nucleotide excision repair pathway were evaluated for associations with lung cancer risk in 203 lung cancer cases and 205 controls and in a case-only analysis of 177 lung cancer patients. Significant relationships between predicted high CYP1A2 activity, CYP1B1 (*1/*3 or *3/*3), GSTM3*A/*A, and XPD (Lys/Gln or Gln/Gln) genotypes and lung cancer risk were observed (adjusted ORs, 2.05; 95%CI, 1.13-3.73, 2.6; 95%CI, 1.19-5.69, 1.84; 95%CI, 1.03-3.31, 2.56; 95%CI, 1.45-4.51, respectively). The predicted mEPHX intermediate or high activity genotype also increased risk approximately 3-fold among females. The combined effects of carrying multiple genetic polymorphisms (gene-gene interaction) or of gene-environment interactions, for example, between the CYP1B1 (*1/*3 or *3/*3) genotype and packyear also resulted in significant levels of increased risk and early age onset of lung cancer.
CYP mRNA expression levels were measured in 20 pairs of lung tumor and histologically normal tissues to evaluate the potential for local metabolic activation and as a smoking exposure biomarker. The detection of CYP1B1 and CYP2E1 mRNA expression in lung tissue suggests that local bioactivation of procarcinogens may occur. A relationship between lung CYP2E1 mRNA expression levels and cigarette smoke and/or other environmental carcinogens exposure was observed. The significant increase in the levels of CYP2E1 mRNA expression in lung tumors compared to their corresponding histological normal adjacent tissues among current and former smokers and nonsmokers who were exposed to petroleum and/or other environmental exposures further suggest a mechanistic link between environmental carcinogens exposures and lung cancer development. The results indicate that individual susceptibility to lung cancer determined by endogenous host factors such as genetic polymorphisms in metabolic and DNA repair genes, family history of lung and other cancers, early age onset, and interindividual differences in capacity of local procarcinogens bioactivation could interact with each other and/or interact with individual exposures or other exogenous factors such as cigarette smoke, environmental carcinogens and occupational exposures in modifying lung cancer risk.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-09262002-124546 |
| Date | 27 November 2002 |
| Creators | Lerdtragool, Sutira |
| Contributors | Marjorie Romkes, Ph.D., John W. Wilson, Ph.D., Jill M. Siegfried, Ph.D., Billy W. Day, Ph.D., Phouthone Keohavong, Ph.D. |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu:80/ETD/available/etd-09262002-124546/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Pittsburgh or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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