Establishing precise neuronal connections is crucial for normal brain function. In many parts of the brain, this is accomplished by refining initially diffuse neuronal connections during development. In contrast to our understanding of the mechanisms by which excitatory connections are refined, the refinement of inhibitory circuits is poorly understood.
In this thesis, I investigated the refinement of inhibitory connections in the lateral superior olive (LSO), a mammalian auditory brainstem nucleus involved in sound localization. The glycinergic, and during development also GABAergic, projection from the medial nucleus of the trapezoid body (MNTB) to the LSO is tonotopically organized with a single-cell-level precision in adults. I asked whether and by what mechanisms the precise organization emerges during development.
I found that the refinement of this inhibitory pathway is achieved by a large degree of functional elimination of exuberant inputs and strengthening of maintained inputs. Elimination of inputs occurred in a frequency-specific manner, resulting in a sharper inhibitory topography. The topographic refinement occurred while the MNTB inputs act excitatory, raising the possibility that depolarizing action of GABA and glycine is a mechanism underlying the rearrangement of inhibitory synaptic connections. In parallel with the elimination, synaptic responses elicited by the maintained MNTB inputs increased over 10-fold. This was due to a moderate increase in quantal size and a large increase in the number of release sites formed by each MNTB fiber.
I further investigated whether olivocochlear efferent neurons that project from the brainstem back to the cochlea play a role in the topographic sharpening. I found that the refinement of the MNTB-LSO pathway is impaired in animals with compromised efferent projections. In both efferent-lesioned rats and mice lacking the ?9 nicotinic acetylcholine receptor subunit, LSO neurons received a greater number of weak MNTB inputs than in control animals. These results indicate that normal olivocochlear efferent projections are necessary for the refinement of the MNTB-LSO pathway. I discuss the possibility that the effect of efferent manipulation could be due to altered levels or temporal patterns of spontaneous activity before hearing onset.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-12172004-051717 |
| Date | 22 December 2004 |
| Creators | Kim, Gunsoo |
| Contributors | Stephen D. Meriney, Daniel J. Simons, Guo-Qiang Bi, Elias Aizenman, Laurence O. Trussell, Karl Kandler |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu/ETD/available/etd-12172004-051717/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Pittsburgh or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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