Mitochondrial deficiencies are present in limb skeletal muscle fibers throughout normal aging and can increase with accumulated exposure to reactive oxide species. Exogenous sources of reactive oxide species include cigarette smoke and other environmental toxins. Intrinsic laryngeal muscles are directly exposed to inhaled toxins which may increase the percentage of cytochrome c oxidase deficient fibers over time. Serial sections of posterior cricoarytenoid and control strap muscle were harvested post laryngectomy from 10 males age 54-78 years old. Cytochrome c oxidase, succinate dehydrogenase, and modified Gomori trichrome stains were used to determine the percentage of type I fibers with cytochrome c oxidase deficiency. Staining with myosin antibodies was used to determine fiber type. The posterior cricoarytenoid muscle contained a significantly higher percentage of type I fibers with cytochrome c oxidase deficiency (p=.002, t = 4.939) compared to the control strap muscle. The percentage of cytochrome c oxidase deficient fibers was also significantly correlated (r = +.851; p < .01) with age in posterior cricoarytenoid muscle. The percentage of type I fibers with cytochrome c oxidase deficiency increases with age in the posterior cricoarytenoid muscle and may be due to the accumulated exposure to reactive oxide species over time.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-12182004-122349 |
| Date | 21 December 2004 |
| Creators | Tellis, Cari Michelle |
| Contributors | J. Scott Yaruss, James J. Sciote, John M. Close, Clark A,. Rosen, Kittie Verdolini |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu/ETD/available/etd-12182004-122349/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Pittsburgh or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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