<p> Human ALS patient samples can be converted into motor neurons for the study of ALS pathogenesis <i>in vitro.</i> A major challenge, however is the lack of maturation of the stem cell-derived tissue, representing a major barrier to therapy development. The body of work herein details a series of studies to interrogate the relative maturation of induced pluripotent stem cell derived MNs and determine the effects of brain microvascular endothelial cells on their maturation. Finally, a novel microphysiological system for the modeling of ALS and the blood brain barrier is developed that includes the co-culture of brain microvascular endothelial cells under continuous flow of media with a focus on motor neuron function and vascular infiltration. </p>
| Identifer | oai:union.ndltd.org:PROQUEST/oai:pqdtoai.proquest.com:10231221 |
| Date | 16 May 2017 |
| Creators | Sances, Samuel |
| Publisher | Cedars-Sinai Medical Center |
| Source Sets | ProQuest.com |
| Language | English |
| Detected Language | English |
| Type | thesis |
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