Yeast Env9 is a conserved oxidoreductase involved in lipid droplet biogenesis

Description

<p> Baker&rsquo;s yeast serves as an ideal model to study endomembrane system due to high conservation of its regulation and trafficking between yeast and human. <i>ENV9</i> is a novel gene involved in vesicular trafficking in <i>Saccharomyces cerevisiae.</i> Previous characterization by our laboratory established that <i>ENV9</i> deletion leads to lysosomal defects and that Env9 is localized to lipid droplets (LDs). Our bioinformatics studies show that <i>ENV9</i> is conserved among eukaryotes and is an orthologue of human Retinol Dehydrogenase 12 (<i> RDH12</i>). </p><p> In this study, we show that Env9 is involved in LD biogenesis by positively regulating LD fusion and glycerol-induced LD proliferation. We establish Env9 to be an oxidoreductase <i>in vitro</i> that displays specific oxidoreductase activity towards RDH12 toxic aldehyde substrate. We also show that Env9 oxidoreductase activity requires its conserved functional domains. Furthermore, we show that oxidoreductase activity is essential for the observed cellular function of Env9 and that interactions with <i>ENV10</i> may be required for <i> ENV9</i>-dependent promotion of LD fusion.</p>

Links & Downloads

1. http://pqdtopen.proquest.com/#viewpdf?dispub=1593799

Tags

Cellular biology

Additional Fields

Identifer	oai:union.ndltd.org:PROQUEST/oai:pqdtoai.proquest.com:1593799
Date	22 August 2015
Creators	Siddiqah, Ikha M.
Publisher	California State University, Long Beach
Source Sets	ProQuest.com
Language	English
Detected Language	English
Type	thesis