The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.
| Identifer | oai:union.ndltd.org:Potsdam/oai:kobv.de-opus-ubp:1220 |
| Date | January 2006 |
| Creators | Schmälzlin, Elmar, Walz, Bernd, Klimant, Ingo, Schewe, Bettina, Löhmannsröben, Hans-Gerd |
| Publisher | Universität Potsdam, Mathematisch-Naturwissenschaftliche Fakultät. Institut für Chemie |
| Source Sets | Potsdam University |
| Language | English |
| Detected Language | English |
| Type | Postprint |
| Format | application/pdf |
| Source | Sensors and Actuators B: Chemical. - ISSN 0925-4005. - 119 (2006), 1, p. 251 - 254 |
| Rights | http://opus.kobv.de/ubp/doku/urheberrecht.php |
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