A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
| Identifer | oai:union.ndltd.org:Potsdam/oai:kobv.de-opus-ubp:1223 |
| Date | January 2005 |
| Creators | Schmälzlin, Elmar, Dongen, Joost T. van, Klimant, Ingo, Marmodée, Bettina, Steup, Martin, Fishahn, Joachim, Geigenberger, Peter, Löhmannsröben, Hans-Gerd |
| Publisher | Universität Potsdam, Mathematisch-Naturwissenschaftliche Fakultät. Institut für Chemie |
| Source Sets | Potsdam University |
| Language | English |
| Detected Language | English |
| Type | Postprint |
| Format | application/pdf |
| Source | Biophysical Journal. - ISSN 1542-0086 (online). - 89 (2005), S. 1339 - 1345 |
| Rights | http://opus.kobv.de/ubp/doku/urheberrecht.php |
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