One in eight women in America will develop breast cancer at some point in their lives. Breast cancer is the second deadliest form of cancer for women in the United States. When a suspicious region of the breast is detected, the tissue is diagnosed by removing a sample, preparing an H&E section, and performing histopathology. This procedure is expensive, invasive, and can take days to return a diagnosis. An alternative to excision biopsies is to instead perform an optical biopsy. This work details endomicroscopes intended to perform optical biopsies in breast tissue. The work address two issues limiting current optical biopsy systems: insufficient resolution and inability to reject out of focus light. To improve the resolution of current endomicroscopes, ultra-slim objectives are developed using optical plastics and zero alignment fabrication techniques. These objectives can outperform current alternative endomicroscope objectives in terms of performance across the field of view and chromatic aberration correction, while remaining as narrow as a biopsy needle. Next, an endomicroscope which utilizes structured illumination to perform optical section is designed, tested, and evaluated on ex vivo breast biopsies. The new endomicroscope provides high contrast images by reducing out of focus background light. Finally, an achromatic, ultra-slim objective and the structured illumination endomicroscope are integrated to form an optical biopsy system with improved lateral resolution and axial response. This integrated system is a step forward for in vivo microscopy and cancer diagnoses.
| Identifer | oai:union.ndltd.org:RICE/oai:scholarship.rice.edu:1911/71981 |
| Date | 16 September 2013 |
| Creators | Kyrish, Matthew |
| Contributors | Tkaczyk, Tomasz S. |
| Source Sets | Rice University |
| Language | English |
| Detected Language | English |
| Type | thesis, text |
| Format | application/pdf |
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