This study focuses on the application of 193-nm excimer laser (ArF) photodissociation to tandem time-of-flight mass spectrometry. In particular, it focuses on identifying the optimal experimental conditions for peptide sequencing and applying the technology to interesting systems. The early focus is on optimizing the sample preparation conditions that define the initial internal energy state of MALDI-produced ions. Subsequent chapters investigate the effect of changing photodissociation laser conditions and define conditions under which the information content of the spectrum is maximized. Later chapters compare the photodissociation experiment to technologies that represent the current state of the art in tandem mass spectrometry, illustrating both the advantages and shortcoming of photodissociation TOF methodology. Finally, we apply photodissociation to the study of interesting systems of biological relevance, including (1) peptides derived from enzymatic digestion, (2) post-translationally modified peptides, and (3) peptide-transition metal ion complexes. In the final chapter we consider the analytical implications of the work as a whole and comment on the analytical viability of the methodology and look forward to new directions for the experiments.
| Identifer | oai:union.ndltd.org:TEXASAandM/oai:repository.tamu.edu:1969.1/1197 |
| Date | 15 November 2004 |
| Creators | Hettick, Justin Michael |
| Contributors | Russell, David H., North, Simon W., Summers, Max D., Vigh, Gyula |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Electronic Dissertation, text |
| Format | 7246930 bytes, 300849 bytes, electronic, text/plain, application/pdf, born digital |
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