The freshwater planarian Schmidtea mediterranea has been used in research for over 100 years, and is an emerging stem cell model. Exteriorly, planarians are covered in mucous secretions of unknown composition. While the planarian genome has been sequenced, it remains mostly unannotated. The goal my master’s research was to annotate the planarian proteome and mucous sub-proteome. Using a proteogenomics approach, I elucidated the proteome and mucous subproteome via mass spectrometry together with an in silico translated transcript database. I identified 1604 proteins, which were annotated using the Swiss-Prot BLAST algorithm and Gene Ontology analysis. The S. mediterranea proteome is highly similar to that predicted for the trematode Schistosoma mansoni associated with schistosomiasis. Remarkably, orthologs of 119 planarian mucous proteins are present in human mucosal secretions and tear fluid. I suggest planarians have potential to be a model system for parasitic worms and diseases underlined by mucous aberrancies.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/33342 |
| Date | 21 November 2012 |
| Creators | Bocchinfuso, Donald Gerald |
| Contributors | Moran, Michael |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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