Bone morphogenetic protein-2 (BMP-2) plays a critical role in bone formation.
The aim of this study was to compare the activity of the mammalian cell BMP-2 to the E-coli cell BMP-2.
In vitro, the potency of mammalian and E-coli BMP-2 was compared by adding BMP-2 to C2C12 cells and measuring the level of alkaline phosphatase activity. In vivo, the activity was evaluated by placing the bioimplants in the thigh muscle of mice, and measuring the amount of bone induced.
The in vitro assay clearly showed that mammalian BMP was significantly more potent than E-coli BMP. In vivo, on the calcium phosphate carrier, mammalian BMP produced more bone than E-coli BMP, but E-coli BMP produced higher density tissue than mammalian BMP. On both mammalian and E-coli BMP, the calcium phosphate carrier had a significant effect on the density but not the quantity of bone produced versus the absorbable collagen sponge carrier.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/42817 |
| Date | 22 November 2013 |
| Creators | Di Lullo, Sylvie |
| Contributors | Peel, Sean, Clokie, Cameron |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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