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Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Platform

This thesis presents the development of a digital microfluidic system to achieve automated sample preparation for the vitrification of mammalian embryos for clinical in vitro fertilization (IVF) applications. This platform included micro devices fabrication, an imaging system, a high voltage control system, and a LabVIEW interface. Individual micro droplets manipulated on the digital microfluidic device were used as micro-vessels to transport a single embryo through a complete vitrification procedure. The device showed cell survival and development rates of 77% and 90%, respectively, which are comparable to the control groups that were manually processed. Technical advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
Date04 July 2014
CreatorsPyne, Derek
ContributorsSun, Yu
Source SetsUniversity of Toronto
Detected LanguageEnglish

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