archives@tulane.edu / Vascular endothelial growth factor A (VEGFA) is a key contributor to the formation of
new blood vessels and angiogenesis is commonly seen in wound healing, cancer, and
inflammatory diseases. However, whether interleukin-17 (IL-17) can induce the
expression of VEGFA in prostate cancer cells remains unknown. In this study, Western
blot analysis and reverse transcription-polymerase chain reaction (RT-PCR) analysis
validated that expression of VEGFA in human prostate cancer LNCaP cells under IL-17
treatment was significantly higher than the untreated control group. The conditioned
culture medium (CM) of LNCaP cells treated with IL-17 increased tube number, tube
nodes, and tube length formed by human umbilical vein endothelial cells (HUVEC) in
tube formation assays, compared with the control CM without IL-17 treatment.
Collectively, these findings reveal that the expression of VEGFA is induced by IL-17 in
LNCaP prostate cancer cells, which leads to increased angiogenesis of HUVEC cells.
This study suggests that expression of VEGFA may be up-regulated by IL-17 in prostate
cancer to enhance tumor angiogenesis. / 1 / Benyu Li
| Identifer | oai:union.ndltd.org:TULANE/oai:http://digitallibrary.tulane.edu/:tulane_93574 |
| Date | January 2019 |
| Contributors | Li, Benyu (author), You, Zongbing (Thesis advisor), School of Medicine Biomedical Engineering (Degree granting institution) |
| Publisher | Tulane University |
| Source Sets | Tulane University |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | electronic, pages: 47 |
| Rights | No embargo, Copyright is in accordance with U.S. Copyright law. |
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