碩士 / 國立中山大學 / 海洋生物研究所 / 81 / Dopamine has a inhibitory effect on the secretion of GTH.
However, it is not clear whether the inhibtory effect of
dopamine is directory acting on the gonadotroph or indirectly
through the GnRH system. The purpose of this study is to set up
a radioimmunoassay (RIA) to determine the concentration of
salmon GnRH and to study the regulatory function of
dopaminergic system on in vitro GnRH release. The synthetic
salmon GnRH (sGnRH) was conjugated to bovine serum albumin
(BSA) using bis-diazotized benidine (BDB) as a bridge and the
sGnRH-BDB-BSA complex was used as a immunogen tonduce antibody
for the RIA. Anti-sGnRH serum at the titer of 1:120,000 had a
cross-reactivity with mammalian GnRH and chickennRH-I of 60.68%
and 144.12% respectively, but had no significantross-reactivity
with other peptides (TRH, Isotocin,g-vasotocin, Somatostatin).
The replacement curve of sGnRHparallelism to mGnRH and cGnRH-I.
Twenty-eight sex quiescenttilapia (Gonadosomatic index=0.125
±0.06%) were sacrificede in vitro study of GnRH secretion.
GnRH release from the-anterior hypothalamus (P-AH) and
pituitaries in responsedopamine (2 hr), 10.8 uM pimozide (1 hr)
and 60 mM K+ (1monitored at 15 minutes intervals. The results
indicateddtable GnRH is released in vitro from both P-AH
regionries in tilapia. However, there are no significantean
concentration in response to dopamine orexperiment suggests
that the dopaminergic systemant inhibitory function on GnRH
secretion of malequiescent.
| Identifer | oai:union.ndltd.org:TW/081NSYSU270009 |
| Date | January 1993 |
| Creators | Chen, Inne Lian, 陳瑩蓮 |
| Contributors | Kao, Chen Chih, 高承志 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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