碩士 / 國立臺灣大學 / 漁業學系 / 81 / Two anionic isoenzymes of glutathione S-transferase (EC
2.5.1.18 ), GST θ and GST μ, have been purified from the eyes
of the shrimp Penaseus japonicus by using a combination of S-
hexyl- glutathione affinity column chromatography and Mono Q
fast protein liquid chromatography (f.p.l.c.). The purified
GST θ was a dimeric enzyme protein with subunit Mr abount 25
kDa. HPLC reverse phase C4 column analysis of various shrimp
tissues indicated that the ratio of GST θ vs. GST μ was
highest in the eye and gill. HPLC reverse phase C4 column
analysis demostrated that corticosterone was selective binding
to GST θ. Glutathione influenced the binding of corticosterone
to the GST θ.
| Identifer | oai:union.ndltd.org:TW/081NTU00453007 |
| Date | January 1993 |
| Creators | Tseng,Shu-Fen, 曾淑汾 |
| Contributors | Chuang,Nin-Nin;Lo,hsiu-wan, 莊寧寧;羅秀婉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 56 |
Page generated in 0.0016 seconds