碩士 / 國立中興大學 / 植物學系 / 82 / From different stage of the cell cycle using synchronous
culture method, Chlorella sp. strain DT cells were desiccated
to dry and recultured in liquid medium. Physiological activity
of cells from the beginning periods cell cycle was restored.
The pigments of the fresh cells and desiccated cells sampled
from different time of the cell cycle were analyzed. Only the
desiccated cells from 14th time had a higher level of
zeaxanthin. There were no differences on the level of
chlorophyll and caroteniods between fresh cells and desiccated
cells. It means that, under desiccated condition, zeaxanthin
may play a role in protecting these pigments from injury.
However, zeaxanthin is not responsible to the regrowing of DT
cells from desiccation stage. Gel electrophoresis found a new
250 kD protein appeared in the desiccated cells from
nonsynchronous culture. A 150 kD soluble protein and a 53 kD
membrane protein also appeared in the desiccated cells. It is
believed that these proteins may not play a role in the
regrowing process of DT cells. At 8th, 12th, 14th, 18th hour of
cell cycle, some membrane protein contents decreased. But at
the other time of cell cycle, the same situation was not
happened. It is indicated that at 4-18 hr of cell cycle, cells
must be more sensitive to desiccation. Cells can not maintain
the protein content in the cell. So cells at these time can not
regrow after desiccation.
| Identifer | oai:union.ndltd.org:TW/082NCHU0366005 |
| Date | January 1994 |
| Creators | Ming-Larng Hwang, 黃敏郎 |
| Contributors | Pei-Chung Cheng, 陳伯中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 60 |
Page generated in 0.0298 seconds