碩士 / 國立中興大學 / 獸醫學系 / 82 / The aims of this study are to evaluate the efficacies of 11 gl-
ycans（Bar, curdlan, dextran sulfate, inulin, krestin, laminar-
an, levan, PO, scleroglucan, yeast glucan, and zymosan） to pr-
otect four different species of fish, bighead carp（
Aristichthys nobilis), grass carp (Ctenopharyngodon idellus),
milk fish (Cha- nos chanos), and tilapia (Tilapia aureas P.)
against bacterial infections in vivo. The result revealed that
several glycans, Bar, krestin, sclerog- lucan, and zymosan, did
significantly protect the four kinds of fish against the
bacterial infections. At the same time, the data shown that the
antibacterial mechanism of glycans associat- ed with the
activation of the nonspecific phagocytosis. The results of
challenging with Aeromonas hydrophila study sugg- ested that
four glycans, Bar, krestin, scleroglucan, and zymosa- n,
significantly increased the survival rates in grass carp（6
0﹪, 70﹪, 90﹪, and 60﹪） and tilapia（80﹪, 60﹪, 70﹪, and
60﹪ five glycans, Bar, curdlan, krestin,scleroglucan,and
zymos- an,did significantly increase the survival rates in both
bighead carp（70﹪, 40﹪, 40﹪, 70﹪, and 50﹪） and milk fish
（50﹪, , 40﹪, 60﹪, and 40﹪）. The results of challenging
with Edwardsiella tarda study indicated that four glycans, Bar,
krestin, scleroglucan, and zymosan, did significantly raise the
survival rates of all fishes. Studing on the kinetics of the
numbers of nitroblue tetrazolium (NBT）- positive staining
cells by the Adherence/NBT Assay eva- luates the non-specific
immunity responses in the fish injected with the five glycans
including Bar, curdlan, krestin, sclerogl- ucan, and zymosan,
by the dose of 10mg/kg bw, respectively. The result revealed
that those five glycans, except curdlan in milk fish, did
significantly increase the numbers of NBT- positive staining
cells in the four species of fish.
| Identifer | oai:union.ndltd.org:TW/082NCHU0541006 |
| Date | January 1994 |
| Creators | Wang Duen Horng, 王敦弘 |
| Contributors | Wang Way Shan, 王渭賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 106 |
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