碩士 / 國立中興大學 / 植物病理學研究所 / 82 / The purpose of this study was to make investigation into the distribution, physiological characters and pathogenicity on Fomitopisi pinicola (Sw.:Fr.) Karst.The fungus was often found above the 1900-meter-high mountains in Taiwan,and it was parasitic or saprophytic mainly on Tsuga chinensis, partially on Picea morrisonicola. The fruitbodies, whose upper surface color was pale yellowish, reddish orange, or blackish, perennial, sessile, often reddish and resinous at margins. Hyphae dimitic system with a dominance of skeletal hyphae. Spores were cylindrical to oblong-elliptical, hyaline, smooth thin-walled, 5.5-8.×3.0-4.0□m. The fungal colonies, the marginal hyphae of which were rather distant, were white cottony or cottony-woolly. Generative hyphae were hyaline, thin-walled, with clamps and there were few chlamydospores. Skeletal mycelia thick-walled, aseptate, rarely branched. Crystals produced in the aerial mycelia and in culture media were observed. Mycelial dry weight reached the maximum after 20 days of incubation at 28°C. The temperature ranging from 24 to 32°C was suitable for mycelial growth. The optium temperature for mycelial growth was at 28°C, and no growth occurred above 36°C. The optimum mycelial growth was at pH3-4. Slow mycelial growth occurred above pH 5.5. The optimum water content of sawdust medium for mycelial growth was 45-65%, and below 25% retarded mycelial growth. The best carbon source for fungus growth was dextrin. Mycelia hardly used cellobiose and cellulose as carbon and couldn't use sodium oxalate. Mycelia could efficiently use ammonium sulfate and ammonium tartrate. The utilization of ammonium nitrate and ammonium carbonate was significantly different among the isolates. Mycelia couldn't use nitrite salts. Mycelial dry weight was at its maximum when C/N ratio was 80. The application of dextrin as a carbon source and ammonium sulfate, ammonium tartrate, or ammonium carbonate as a nitrogen source in Czapek's medium could greatly enhanced mycelial grow. The oven dry weight of woods collected from T. chinensis, Abies kawakamii, and P. morrisonicola were drastically reduced 30 days after of the test fungus inoculation. The average dry weight loss was 42.5% in A. kawakamii, 33.6% in T. chinensis, and 32.9% in P. morrisonicola; 120 days after inoculation. With the decaying time increasing, three timbers' color became darker and darker, and the wood texture became very fragile. Decaying potency among the tested isolates were very different , above all, FPJD01 possessed the most powerful ability to deacy. F. pinicola could produce amylolytic enzyme, protease, lipase, cellulase , peroxidase in media. The mycelia in pure culture were not able to excrete polyphenol oxidase, so F. pinicola was one of brown rot fungi.
| Identifer | oai:union.ndltd.org:TW/082NTPU3363009 |
| Date | January 1994 |
| Creators | Yu, Yuan-Horng, 余元宏 |
| Contributors | Han, You-Hsin, 韓又新 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 55 |
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