碩士 / 國立臺灣大學 / 醫事技術學系 / 82 / The thalassemia syndrome,which is caused by absent or defec-
tive synthesis of globin chains,is one of the commonest heredi-
tary hemolytic disorders in Taiwan. Normally .alpha.- and.beta.
- globin chains are synthesized coordinately. Unbalanced globin
chains synthesis caused early destruction of red cells. .alpha.
-thalassemia is characterized by defective .alpha.- globin pro-
duction(.beta.- globin excess),and .beta.-thalassemia by redu-
ced .beta.- globin synthesis(.alpha.- globin excess). In this
study,we developed a non-isotope method to detect the relative
amount of .alpha.- and .beta.- globin mRNA by using the polyme-
rase chain reaction to amplify cDNA copies of circulating reti-
culocyte messenger RNA(mRNA). The relative quantitation was
per- formed by fluorescence measurement of the fluorescent dye
(FAM Amidite) labeled primers incorporated in specific globin
cDNA band on GeneScan 672 software. The results showed thaat
the ra- tios of .alpha./.beta. - globin mRNA after RT-PCR of
.beta. - thalassemia patients ( minor:1.69±0.12, major:5.52
±2.51 )were greater than that of normal adults( 1.49±0.05 ).
Conversely, patients with .alpha. - thalassemia minor showed a
decreased ratio of .alpha./.beta. - globin mRNA( 1.35±0.09 ).
All data showed significant differences from normal control.
Not only the method of detection of the .alpha./.beta. ratio is
a safe and simplified approach toward the screening of
thalassemia syndromes,but also be useful for studying the
globin gene expression,regulation and in monitoring the effects
of the treatment of thalassemia.
| Identifer | oai:union.ndltd.org:TW/082NTU00527001 |
| Date | January 1994 |
| Creators | Chang,Yu-Wen, 張郁玟 |
| Contributors | Lin,Kuo-Sin;Lin,Liang-In, 林國信;林亮音 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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