碩士 / 國立中興大學 / 獸醫學系 / 83 / The acute toxicity of malachite green to rainbow trout was
studied by 1) determining the median lethal concentration
(LC50), 2) histopathological observation of tissue
sections, 3) monitoring the serum biochemical composition and
4) assaying for malachite green concentration in the fish
muscle.The LC50 of malachite green for the six groups of
rainbow trout with average body length of 5.4 cm, 7.9 cm, 8.9
cm, 9.5 cm, 13 cm and 19.7 cm were found to be 0.36 ppm, 0.69
ppm, 0.65 ppm, 0.64 ppm, 1.03 ppm and 0.96 ppm, respectively.
Histopathological examination of the gills of malachite green
exposed fish revealed the swelling of both the primary
and secondary lamella, increased mucus secretion and the
vacuolation of the epithelial cells. Epithelial cells of the
renal tubules showed cloudy swelling. However, no apparent
lesion was observed in the liver. Serum enzymes, such as
aspartate aminotransferase (AST), alanine aminotransferase
(ALT), lactate dehydrogenase (LDH), and blood urea nitrogen
(BUN) were seen to be significantly elevated (P<0.05) in
rainbow trout which had been exposed to a concentration of 0.5
or 1 ppm of malachite green for either 24 or 48 hr. The
concentration of malachite green in the muscle tissue
increased proportionately with the exposed water
concentration, resulting in the muscle malachite green
concentration being several fold higher than that in the
water. This showed that the malaclite green can accumulate
in fish. However, it was also observed that the higher the
fish density in the tank, comparatively lower
malachite green concentration were detected in the fish
muscle. Moreover, the muscle of survivors were found to
contain lower malachite green concentration at 48hr
postexposure (PE) than the fish which died within 12hr PE
despite being exposed to the same treatment concentration.
| Identifer | oai:union.ndltd.org:TW/083NCHU0541002 |
| Date | January 1995 |
| Creators | Luen Goang Lin, 林倫廣 |
| Contributors | Shu Perng Ho, 何素鵬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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