碩士 / 國立臺灣大學 / 生化科學研究所 / 83 / Three clones containing the inserts of 0.55、0.6 and
1.3 kb respectively, were isolated from a carp ovarian cDNA library. Nucleotide sequence analysis revealed that they are
all homologous to the members of cystatin family of other
vertebrates although they differ from each other. The open
reading frames of all these cDNAs encode a protein of 129
amino acids which include a putative signal peptide of 18-20
amino acid residues. In addition, they also contain four
invariable cysteine residues and three highly conserved regions
found in cystatin family. The above data suggest that all
the three cDNAs encode a polypeptide which belongs to the
cystatin family of cysteine proteinase inhibitor.
In this paper, the three cDNAs were also used as probes
to detect the mRNAs expressed in carp tissues. In addition,
they were constructed to an expression vector and expresse
in E.coli. The expressed proteins were used as antigens
to induce antibodies in rabbits, with which the distribution
of these proteins in carp tissues or body fluids were investigated.The results indicate that both cystatin mRNAs and proteins are tissue-specifically expressed only in the ovary. The physiologicalroles of these proteins in carp remain to be investigated.
| Identifer | oai:union.ndltd.org:TW/083NTU02103012 |
| Date | January 1995 |
| Creators | Li, Zhao Chang, 李肇昌 |
| Contributors | Zhang, Yue Xia, Huang, Huo Lian, 張月霞, 黃火鍊 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 44 |
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