碩士 / 國立臺灣大學 / 生化科學研究所 / 83 / In this study, the nucleotide sequences of carp ZP2 cDNA were
determined and their amino acid sequences were deduced. The deduced carp ZP2 contains 12 to 25 glutamine-rich tandem repeats of 12-13 residues in the N-terminal region followed by a sequence homologousto winter flounder and mammalian ZP2 in the C-terminal region.
Northern blot analysis showed that ZP2 mRNA is only present in the ovary but not present in other tissues including liver、testis、head-kidney、kidney and brain. By Western blot analysis, ZP2 was only detected in the vitellogenic oocytes but not in the previtellogenic oocytes, nor in the plasma of estradiol-treated carp. All these results suggest that carp ZP2 is synthesized in the ovary. In addition, the carp ZP2 was found to play roles in the cross-linking of egg membrane components during egg maturation, ovulation and cortical reaction.
| Identifer | oai:union.ndltd.org:TW/083NTU02103013 |
| Date | January 1995 |
| Creators | Xu, Jia Zhang, 徐佳璋 |
| Contributors | Zhang, Yue Xia, Huang, Huo Lian, 張月霞, 黃火鍊 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 54 |
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