碩士 / 國立中山大學 / 海洋資源學系 / 84 / Symbiosis is a ubiquitous phenomenon in marine environments.
Many invertebrates were found to be associated with symbiotic
bacteria. However, these bacteria were difficult to be
cultivated,therefore, limited our understanding toward them.
Previously,their presence could only be observed under
microscope,beyond that hardly any further studies could be
done. The invention of PCR(polymerase chain reaction) technique
circumvented the necessity of bacterial cultivation in order to
study the bacterial composition and their phylogeny. Cinachyra
australiensis was chosen in this thesis. The bacteria co-
existed with the sponge were collected by centrifugation and
their partial 16S rDNA sequences were amplified by PCR. The
nucleic acid sequences were determined and used as the basis of
phylogeny. Meantime, 20 of the cultivable bacteria isolates
were chosen and analyzed by the same protocol as a basis for
comparison. In the cultivable part, 3 isolates were closed to
the Cytophaga-Flavobacter group, 5 were related to Bacillus, 5
belonged to Enterbacteriaceae(including Aeromonas、Altermonas
and Vibrio), 4 were identified as gamma proteobacteria, 2 were
judged as phototrophs(Rhodobium and Rhodopseudomonas) and one
marine Eubacteria sp. As for the uncultivated part (including
cultivable and noncultivable bacteria), thirteen of the 21
clones studied were identical in the sequences analyzed, which
were related to Solemya reidi bacteria symbiont. The other two
indentical clones were also closes to S. reidi symbiont. Two of
the clones, although not indentified, were related to the
Riftia pachyptila trophosome symbiont. The other isolate was
related to the Inanidrilus leukodermatus symbiont. Over all,
85% of the cloneds were related to sulfur-oxidizing symbionts
indicating that Cinachyra australiensis may actually harbor
sulfur-oxidizing bacteria symbionts. However, the nature of the
symbiosis is yet to determined.
| Identifer | oai:union.ndltd.org:TW/084NSYSU277014 |
| Date | January 1996 |
| Creators | WANG, YI KAI, 王憶鎧 |
| Contributors | HSU, CHI HSIN, 徐基新 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 117 |
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