碩士 / 國立海洋大學 / 水產養殖學系 / 84 / In order to study the antivrial machanism induced by poly I:C,
we firsttested and found that the poly I:C dose not cause
cytotoxicity to fishcell lines. We treated 5 different fish cell
lines with poly I:C thentreated with T42G strain IPNV, and found
that RTG-2 cell line preasenthighest antiviral ability in
response to the poly I:C treatment. Whendifferent
concerntractions of poly I:C were applied to the RTG-2 cells,the
antiviral ability of these cells increased as the dosage of poly
I:Cincreased, up to 6 ug/ml of poly I:C. SDS-PAGE
electrophoresis to analysisthe poly I:C treated RTG-2 cell
culture medium showed the present of an extra-protein band of
about 17.4KD by commassie blue staining. In order to confirm
that this protein is induced by poly I:C, we labled the poly I:
Ctreated RTG-2 cells with 35S-methionine,and showed that a
labled protein of about 17.4KD also present in the culture
medium, and the radioactivity ofthis protein band is stronger
than the one in the medium of control not treated with poly I:C.
So we proved that this protein is induced by poly I:Ctreatment.
According to the above data, we think that the interferon gene
is exist inthe RTG-2 cells, so we synthesized squence-specific
primers according to thecDNA squence of flatfish interferon
published by Tamai, to amplify and squenced, which showen 48%
homology when compared with flatfish interferon cDNA. Since
there are stop codens with in the fragment. We need further
studyto evaluate whether this fragment really respresent a part
of fish interferongene.
| Identifer | oai:union.ndltd.org:TW/084NTOU0086016 |
| Date | January 1996 |
| Creators | Wu, chieng-hung, 巫建宏 |
| Contributors | Chou Hsin-Yiu, 周信佑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 54 |
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