碩士 / 國立臺灣大學 / 動物學系 / 84 / The promoter trapping method is introduce a promoter-less
reporter gene to integrate onto the down stream of a activated
promoter or an expression gene as well as to express the
reporter gene. Through the activation of selecting gene,
Neomycin phosphotransferase(Neo), cells with the integrated
gene survived under the selection of G418.Most studies of
promoter trapping methods were focused on the mouse embryonic
stem cells. By applying this technique, the genes involved in
the embryonic development were trapped, which is pretty helpful
for the function analysis of mouse genes. Up to now, no study
of promoter trapping was repoted on fish cells. The objective
of our research is to study the promoter trapping method
applied on fish cells.We used the tilapia ovary cell (To-2) as
material and designed a promoter trapping vector to work on the
TO-2 cells. The aims of our study are to trap promoters, to
increase the success of trapping, and ultimately, to find out
possibilitty to apply this technique on fish embryonic stem
cells.
| Identifer | oai:union.ndltd.org:TW/084NTU00312017 |
| Date | January 1996 |
| Creators | Chi-Min Kao, 高啟明 |
| Contributors | Lo, Chu-Fang; Chang, Chi-Yao, 羅竹芳,張繼堯 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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