碩士 / 國立臺灣大學 / 漁業學系 / 84 / This is the first research ever attempting to isolate two
components of paralytic shellfish toxins from Alexandrium
minutum Halim in Taiwan. The two toxins were obtained by
means of two different yet successive preparative
chromatography on the water soluble extract that taken from a
mass culture of Alexandrium minutum Halim AM-1. The sample
was first seperated by a gel filtration chromatography,
then the toxin fraction obtained was then puried by an linear
gradient elution with 0-0.025N acetic acid and a subsequent
0.027-0.03N acetic acid elution on the toxin fraction.
Gonyautoxin-1 and Gonyautoxin-4 were thus purified and
confirmed by 1H-NMR spectroscopic analysis. There exist two
other minor toxins which have been confirmed yet not
purified in A. minutum are Gonyautoxin-3 and
Gonyautoxin-2. Solution of pure Gonyautoxin-1 and
Gonyautoxin-4 were found loosing their nature gradually
within 12 days even in pH=3.3 acetic acid solution and a
temperature below -20℃. Pure form of toxins was also found to
degrade or to convert to its epimer in a solution of higher pH
or higher temperature. A drying procedure such as
lyophilization may cause degradation and interconversion. The
second portion of this research is the routine monitoring
result of paralytic shellfish toxins in the culturing
shellfishes along the south western coast of Taiwan during the
year of 1993- 1995. Among the three man-raised shellfishes,
clam, oyster and purple clam, only purple clam was found to be
toxic occasionally. It was also found that almost all the
toxic purple clams were from Pintung area. The toxic purple
clams were found to contain Gonyautoxin-1, Gonyautoxin-2,
Gonyautoxin-3, and Gonyautoxin-4 which were the same
composition of toxins found in A. minutum collceted in the
same area. Sometimes, the toxicity of purple clam may exceeded
the quarantine allowance of U.S. or Japan by
| Identifer | oai:union.ndltd.org:TW/084NTU00453001 |
| Date | January 1996 |
| Creators | Jia-Wei Chang, 張家維 |
| Contributors | Hong-Nong Chou, 周宏農 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 84 |
Page generated in 0.0159 seconds