碩士 / 國立臺灣大學 / 漁業學系 / 84 / To investigate the molecular structures of the visual pigment
genes and the initial biochemical reactions involved in the
visual signal transduction relevant to fish physiology, the
sequence of the visual pigment gene has to be determined. A
putative cDNA encoding carp type I rhodopsin (Tsai et al.,
1994) and a partial cDNA encoding type II rhodopsin (Chong,
1994) had been cloned from a retinal cDNA library. In the
present work, the completely sequenced and analyzed. The
deduced amino acid sequence of type II rhodopsin reveals that
there are only five amino acid residues differences between
type I and type II, i.e., (i) the 19th residue in type I is
Val, instead of the Ile in type II (Val-19-Ile), (ii) Ile-54-
Val, (iii) Ile-108-Val, (iv) Val-169-Glu, and (v) His-315-Asn.
Besides, there are 4 polyadenylation signals in type II instead
of 1 in type I, and the 3' untranslated region of type II is 99
bases longer. Furthermore, a genomic library of the common carp
was constructed and the genomic DNA of type II rhodopsin was
isolated. The genomic DNA was sequenced and the primer
extension assay was performed to determine the transcription
start site. The results reveal that (1) the gene is not
interrupted by any intron, (2) the initiation of the
transcripts of rhodopsin II is similar to that of other
vertebrates, and (3) there are conserved regions and retina-
specific nuclear protein binding sites found in the upstream
region. The Southern analysis of genomic DNA and the reverse
transcriptase-polymerase chain reaction imply that the two
types of rhodopsins are from different individuals of the same
population. On the other hand, a clone (3aV1) which resembles
the retinal epithelium-specific protein gene was screened from
the genomic library using the partial cDNA of green opsin as a
probe. The results indicate that a fragment of the deletion
clones of 3aV1 is 66% identical to the human satellite
| Identifer | oai:union.ndltd.org:TW/084NTU00453006 |
| Date | January 1996 |
| Creators | Lim Jormay, 林若梅 |
| Contributors | Tsai, Huai-Jen, 蔡懷楨 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 69 |
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