碩士 / 國立中興大學 / 植物學系 / 85 / Desiccation-tolerant Chlorella sp. strain DT(from an arid
mountainous area of Taiwan) and Desiccation-sensitive Chlorella
pyrenoidosa (211-8b, from the AlgaeCollection Center, Univ,
Gottingen, Germany) were cultured Chlorella-medium.Only a
limited amount of water can be conserved in the DT and 8b cell
when dehydrate using an infra-red radiation balance at 42oC for
3 h, and it tookonly 130min to dehydrate the Chlorella cells
completely when heated at 60oC. The dried cells stored in
desiccation box for 3 days, the DT cells dehydrate 42oC could
restore growth absolutely after 7-8 days rewetting in liquid
culturemedium. The DT dehydrate at 60oC and the 8b dehydrate at
42oC or 60oC could not restore growth.Two-dimensional gel
electrophoresis analysis of polypeptides revealed that
thepatterans of fresh and dehydrated at 42oC differed
significantly in their polypeptide profiles. As shown in the
figure, approximately 280 polypeptides could be resolved by the
2-D system. The changes included increase, decrease ornewly
induced synthesis. In general, labelling patterns were similar
despite sample differences.The difference became conspicuously
after desiccation DTrestored growth in liquid culture medium.
| Identifer | oai:union.ndltd.org:TW/085NCHU0366025 |
| Date | January 1997 |
| Creators | Peng, Yu-Wen, 彭雨文 |
| Contributors | Pei-Chung Chen, 陳伯中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 9 |
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