碩士 / 國立海洋大學 / 海洋生物研究所 / 85 / Abstract
The specific protein in chorion, a resistant acellular coat in
fish eggs, is a glycoprotein and has a role on identifying
the sperms from the same species for fertilization. The
glycoprotein in chorion of unfertilized eggs of Epinephelus
awoara was primary isolated and purified by SDS-PAGE and
gel filtration chromatography for further cytochemically
studying in the cellular location and biosynthetical
mechanism of the protein. Effects of temperature, pH and
salt concentration of the soaking buffer on the release of
glycoprotein from the egg surface were studied. Periodic acid-
silver stain was used for identifying glycoprotein. The
principal glycoproteins in unfertilized egg chorion of E.
awoara were 78 kD, 52 kD and 35 kD. Among these
proteins the amount of 78 kD was the highest, 52 kD was the
next. With using gel filtration chromatography 78 kD showed four
proteins. However, 52 kD may be the female-specific
glycoprotein on the egg surface. As temperature,
pH or salt concentration of the soaking buffer was changed
the three proteins were easily released from the chorion without
breaking eggs. The results suggested that the three
glycoproteins were not integral membrane proteins in the
fish eggs. Anyway, the female-specific protein will be
further studied molecular structure and in vivo location by
using immunocytochemical technique.
| Identifer | oai:union.ndltd.org:TW/085NTOU0270004 |
| Date | January 1997 |
| Creators | Ding, Shu-Hui, 丁淑慧 |
| Contributors | Jui-Sen Yang Ph.D., 楊瑞森 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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