碩士 / 國立臺灣大學 / 動物學系 / 85 / c-mos基因產物是個蛋白, 在脊椎動物之卵成熟過程中調節各項重要事
件. 就蛙卵而言,Mos蛋白激負責誘發GVBD (germinal vesicle
breakdown), 於第一次和第二次減數分裂過渡時抑制DNA合成, 並維持成
熟卵靜止於第二次減數分裂中期. Mos蛋白激之作用機制與MPF
(maturation-promoting factor)作用有著密不可分之關係, 與Mos蛋白質
交互作用之分子更結合成一複雜之交織. 此多元化之分子性質, 可能歸因
於Mos蛋白質合成初期不穩定之性質與磷酸化狀態而達成. 對於Mos蛋白之
生理研究, 多採融合蛋白進行研究. 鯉魚卵成熟的樣態與蛙卵系統有所不
同, 但鯉魚卵成熟的研究僅只於MPF, 尚缺乏完整分子機制之構圖. 本實
驗室則用已選殖到的鯉魚c-mos基因, 利用分子生物學技術, 促使細菌表
現c-mos非融合蛋白, 以極溫和之純化步驟獲取較近於內生性之Mos蛋白,
並針對此蛋白作了一些基本性狀探討及與魚卵萃取液之蛋白質之交互作
用. 發現到表現的Mos蛋白在純化後去鹽時有消失的現象. 並且發現到細
菌表現蛋白為一磷酸蛋白, 且於表現之初便已呈磷酸化狀態. 這些基本性
狀及初步於卵萃液中之觀察對於未來c-mos蛋白在魚卵成熟分子機制的研
究將會有所助益.
The Mos protein kinase, product of the c-mos proto-oncogene, is
a key regulatorof oocyte maturation in vertebrates. In Xenopus
laevis, Mos protein kinase is involved in inducing germinal
vesicle breakdown, inhibiting DNA replicationdyring meiosis I/
meiosis II transition and maintaining the metaphase arrest of
meiosis II in mature eggs. Maturation promoting factor (MPF)
activities duringoocyte maturation are also regulated by Mos or
Mos-mediated mechanisms. Mosalso interacts with many molecules
other than MPF. The multi-functionalproperties of Mos might be
attributed to its various phosphorylation statesand instable
property. In spite of the apparent conformation problems,
mostresearches in Xenopus oocyte system used Mos fusion protein
as a tool. On thehand, researches in fish oocyte maturation are
in relatively slow progressionwithout incorporating Mos into
studies. We had cloned c-mos gene from the carpgenomic library.
In this study, the c-mos gene product of the carp was expressed
as a non-fusion protein in E. coli. The expressed protein
showeddifferent characters from Mos fusion protein utilized in
the study of Xenopusoocytes. Characterization of the carp c-mos
protein in vitro will help theinvestigation of Mos molecular
mechanism in vivo in the future.
| Identifer | oai:union.ndltd.org:TW/085NTU00312006 |
| Date | January 1997 |
| Creators | Ju, Tsai-Kai, 朱蔡豈 |
| Contributors | Ferng-Chen Ke, 柯逢春 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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