碩士 / 國立臺灣大學 / 漁業科學研究所 / 85 / The freshwater giant prawn（Macrobrachium rosenbergii）is one of
the most important aquatic cultured shellfish in Taiwan. Since
1994, the freshwater giant prawns were often found to be
suffering from white muscle disease in the spring and autumn
seasons. The infected prawns were frequently found around bank
of pond. Diseased prawn reveals symptoms of less activity,
anorexia and white muscle. The prawn died soon after harvesting
which may cause an economic loss for cultured farmers.
Histopathological observation showed that several rod bacteria
were observed in the muscle. 120 strains of bacterial samples
were collected from a period between August to October , 1995
and July to October , 1996 from Likang, Lingro, Chaocho and
Meilong, Taiwan. The results showed that over 95 % the bacteria
strains were Enterobacteriaceae, and 40 % belongs to Citrobacter
freundii. The major characteristics for C. freundii are indole
negative, H2S positive, Lysine decarboxylase negative, citrate
utilization positive, VP test negative, positive on grow of KCN
medium, inability of using Malonate and non-fermentation for
Adonitol. The challenge test showed the lethal dose50（LD50）
was 4 x 106 CFU/g body weights. An experiment performed on
histopathology showed that the bacteria was observed in the
infected muscular tissue . The infected muscuE6 CFU/g body
weights. An experiment performed on histohemocyte encapsulated
nodules were also observed in the necrotic sites of infected
muscle and hepatopancreas. In hepatopancreas , the dilated
sinus and expended tissue fluid and increasing of hemocytes were
found. According to the above results it is therefore suggested
that C. freundii is probably the main cause for white muscle
disease for cultured freshwater prawns.
| Identifer | oai:union.ndltd.org:TW/085NTU00451003 |
| Creators | Lu, Ting-wei, 呂廷威 |
| Contributors | Chen shiu-nan, Kuo ching-ming, 陳秀男, 郭欽明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 55 |
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