碩士 / 國立臺灣大學 / 藥理學研究所 / 85 / The effects of C60 fullerneols 1 and sulfomatobutylate C60
derivated(C60.SO3Na), newer free radical trappers, in protecting
against radical-induced injuries caused by hydrogen peroxide and
cumene hydroperoxide were studied in hippocampal slices of rat
in vitro. Both hydrogen peroxide(0.004-0.008%) and cumene
hydroperoxide (0.005-1 mM) reversibly decreased theamplitudes of
the CA1 evoked population spikes in the hippocampal slices at a
concentration and perfusion time dependent manner. C60
fullerenols 1 and C60.SO3Na both significantly prevented the
hydrogen peroxide (0.006%) and cumene hydroperoxide(1 mM)
elicited decreasing effects of the population spikes. Adenosine
(0.03 mM)and DNQX (0.002 mM) both reduced the amplitudes of
population spikes. Neither C60 fullerenols 1 nor C60.SO3Na alter
the decreasing effects of adenosineand DNQX. A thiobarbituric
acid (TBA) test was used to asses the effect of C60 fullerenols
1 and C60.SO3Na on lipid peroxidation elicited in
hippocampalslices by hydrogen peroxide and cumene hydroperoxide.
Incubation in the presenceof C60 fullerenols or C60.SO3Na
totally prevented the increase in lipidperoxidation by hydrogen
peroxide and cumene hydroperoxide in both the incubationmedium
and the slice homogenates. These results indicated that free
radical trappers like C60 fullerenols 1 or C60.SO3Na prevented
damage produced by hydrogen peroxide and cumene hydroperoxide in
hippocampal slices. The effects may be associated with the free
radical scavenging activities of C60 fullerenols1 and C60.SO3Na.
| Identifer | oai:union.ndltd.org:TW/085NTU00550006 |
| Date | January 1997 |
| Creators | Huang, Kuang-Chih, 黃光智 |
| Contributors | Tsai Min-Chen, 蔡明正 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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