碩士 / 淡江大學 / 水資源及環境工程學系 / 85 / The traditional methods for detecting the amount of polychlorinated biphenyls (PCBs) in fish are mainly to destroy the fish tissue by sponification and then to extract the PCBs by liquid-liquid extraction. This process not only is time- consuming but also requires a large amount of solvent. Therefore, the goal of this study is primary to detect the amount of PCBs in fish using microwave extraction and different cleanup procedures, such as silica, Florisil and alumina, etc. The concentration of PCBs congeners can be determined by using gas chromatography/electron capture detector(GC/ECD) and indicating of quantitative recovery, and comparison of the results between microwave extraction and sponification. The results show that quantitative recovery from microwave extraction is above 80%, which is larger than that of sponification, (75. 6%. )for 10 PCBs congeners.
The procedures for microwave extraction was that temperature was increased to 115℃ in 5 minutes by 80% microwave power and then hold for 10 minutes. The average quantitative recovery of 9 PCBs congeners is 93.2% in certified reference material CARP-1 of fish. Hence, it is evident that the result of microwave extraction is quiet good.
This study is mainly to determine 10 PCBs congeners, i.e., IUPAC No.18, 31, 44, 52, 101, 110, 138, 153, 170, 180, spiked in fish and then to extract PCBs using microwave energy as well as the concentrations of PCBs is in the range of 2.2 to12.3 pg. Finally, we also found that the concentrations of PCBs of the fishes, such as Lizza carinata、Megalops cyprinoides and Tilapia in highly-polluted water (Er-Jen stream)have more than that of PCBs in fish as Channa sp. The amount of 10 PCBs congeners contained in fish from Er-Jen stream are ranging 0 to 172.5 ng/g. Among 10 PCBs congeners, the concentractions of PCB-101、PCB-110、 PCB-153、PCB-180 can be found more than that of PCBs. The concentractions of PCBs congeners of the fishes in Tamshui river are ranging 0 to 16.6 ng/g, Megalops cyprinoides and Tilapia contain more. The mount of PCBs congeners found in others areas can be detected less than 5.81 ng/g.
| Identifer | oai:union.ndltd.org:TW/085TKU03087021 |
| Date | January 1997 |
| Creators | Chuang, Li-Lin, 莊豊霖 |
| Contributors | Hsu, Tau-Being, 許道平 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 138 |
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