碩士 / 高雄醫學院 / 藥學研究所 / 86 / Part I : Studies on the Bioactive Principles of Corydalis
ophiocarpa : The freshroot of Corydalis ophiocarpa (
Papaveraceae ) (1.02 Kg) were chipped and extracted with
methanol. The methanol extract was chromatographed on silica gel
to yield three compounds : protopine (1), palmatine (2),
berberine (3). The structure of these three alkaloids were
determined by spectroscopic methods. The antiplatelet effects of
synthetic products from palmatine(2) and berberine (3),
tetrahydropalmatine (4), and tetrahydroberberine (5), were
studied by using humanplatelet - rich plasma (PRP). In human
citrated platelet - rich plasma, palmatine(2), berberine (3),
tetrahydropalmatine (4) and tetrahydroberberine (5) inhibited
the second phase, but not the primary phase, of aggregation
induced by epinephrine and ADP, and in a concentration -
dependent manner. Protopine (1) in ahigh concentration ( 300 uM
), inhibited the primary phase and the second phaseof
aggregation induced by epinephrine and ADP. These results
suggest that theantiplatelet effects of compound (2)-(5) are
mainly due to inhibition of the formation of thromboxane A2.
Compound (1) is not only due to inhibition of theformation of
thromboxane A2, but also probably increase c-AMP, that
inhibitedthe primary phase of aggregation induced by epinephrine
and ADP. Part II : Studies on the Bioactive Principles of
Marine Invertebrate Animal-Derived Actinomycetes : Two
antibiotics, actinomycin D and X2, were isolated from the
culture brothof an Streptomyces strain 1-19-3, a marine
invertebrate animal-derived Actinomycetales. The structure of
these two antibiotics were determined by spectroscopic methods.
The cytotoxic activity of actinomycin X2 against 212 (ED50 =
0.34 ng/mL ), PLC/ PRF /5 ( ED50 = 0.19 ng/mL ) , HT-3 ( ED50 =
0.18 ng/mL ) , T 24 ( ED50 = 1.24 ng/mL ) , SiHa ( ED50 = 0.61
ng/mL ) , and CaSKi ( ED50 = 1.57 ng/mL ) cells in-vitro was
studied. Actinomycin X2 showed strong cytotoxic activity against
the above cell lines.
| Identifer | oai:union.ndltd.org:TW/086KMC00551004 |
| Date | January 1998 |
| Creators | Lien, Guan-Huei, 連冠惠 |
| Contributors | Mei-Ing Chung, Chun-Nan Lin, 鍾美英, 林忠男 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 135 |
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