碩士 / 國立中興大學 / 獸醫學系 / 86 / The aim of this investigation is to develop a simple, rapid,
and reliablehigh-performance liquid chromatographic (HPLC)
method for the simultaneous determination of the first
generation of quinolones (oxolinic acid, nalidixicacid, and
flumequine) and the second generation of quinolones
(ciprofloxacin,danofloxacin, enrofloxacin, ofloxacin, and
sarafloxacin) in fish, pork, and chicken meat. The HPLC
separation was carried out on a C18 column ( Cosmosil 5C18-MS
column; 150mm x 4.6 mm I.D.) with a guard column with 0.1%
triethylamine buffer(pH 3.0) containing 7 mM sodium dodecyl
sulfate (SDS)-acetonitrile (65:35) asthe mobilephase at a flow
rate of 0.7 ml/min. The chemicals were detected byUV detection
( UV wavelength programming) which wavelength was first set at
254 nm then it was changed to 282 nm immediately by a
programming technique for 11 min. At the same time, the
fluorescence detection was used (excitation wavelength 325nm,
emission wavelength 365 nm, (excitation wavelength 280 nm,
emission wavelength 450 nm). The samples were prepared by
extraction with acetonitrile and methanolicsodium hydroxide
solution flowed by liquid-liquid partition with acetonitrile
saturated n-hexane for clean up. The recoveries of the chemicals
from fish, pork,and poultry meat fortified at the level of
0.02~0.5 ug/g were 73.1~91.7%, withhigh precision. The limit of
detection of our developed method was 0.01 ug/g foreach drug.
Each 50 samples of fish, pork, and chicken meat from market
located in Taiwan area were surveyed by random for the residues
of quinolones with the developed method. The results of the
residues of quinolones in chicken, pork, and fish samples were
2%, 0%, and 0%, respectivately.
| Identifer | oai:union.ndltd.org:TW/086NCHU1541001 |
| Date | January 1998 |
| Creators | Gong, Peir-Shen, 龔培森 |
| Contributors | Wang Way-Shyan, 王渭賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 1 |
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